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L-Type Ca Ion Channel

dhpr_pic.png

Below is a stack (952 MB) of 15,229 images (MRC/CCP4 format) of L-type Calcium channel (DHPR [1]). The images were recorded with a FEI Tecnai F20 electron cryo-mircroscope equipped with a field emission gun operating at 200 kV. Micrographs were taken under low-dose conditions at an underfocus between 3.0 and 4.5 µm and magnification of 62,000x, which were then compressed threefold resulting in a pixel size of 3.39 Å/pixel at the specimen level. Alignment parameters determined by Frealign for each particle in the stack can also be found below, as well as a reconstruction of DHPR (also MRC/CCP4 format; a B-factor of -3000.0 Å2 and 23.0 Å low-pass filter radius were applied using the program bfactor to sharpen the density) and a script to calculate a reconstruction using Frealign.


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