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Movies of ice-embedded particles enhance resolution in electron cryo-microscopy

TitleMovies of ice-embedded particles enhance resolution in electron cryo-microscopy
Publication TypeJournal Article
Year of Publication2012
AuthorsCampbell, MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS, Carragher B, Grigorieff N
Refereed DesignationRefereed
JournalStructure
Volume20
Pagination1823–1828
Date Published9/2012
ISSN09692126
Abstract

Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by beam-induced motion and sample stage instabilities. This procedure increases the efficiency of cryo-EM imaging and enhances the resolution obtained in three-dimensional reconstructions of the particle. Using movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of midrange electron microscopes that may have limited mechanical stability and beam coherence.

DOIhttp://dx.doi.org/10.1016/j.str.2012.08.026
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