Skip to Content

Quantitative characterization of electron detectors for transmission electron microscopy

TitleQuantitative characterization of electron detectors for transmission electron microscopy
Publication TypeJournal Article
Year of Publication2013
AuthorsRuskin, RS, Yu Z, Grigorieff N
Refereed DesignationRefereed
JournalJ Struct Biol
Volume184
Pagination385-393
Date Published2013 Nov 1
ISSN1095-8657
Abstract

A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of their modulation transfer function (MTF) and detective quantum efficiency (DQE). However, the performance of these new detectors needs to be carefully monitored in order to optimize imaging conditions and check for degradation over time. We have developed an easy-to-use software tool, FindDQE, to measure MTF and DQE of electron detectors using images of a microscope's built-in beam stop. Using this software, we have determined the DQE curves of four direct electron detectors currently available: the Gatan K2 Summit, the FEI Falcon I and II, and the Direct Electron DE-12, under a variety of total dose and dose rate conditions. We have additionally measured the curves for the Gatan US4000 and TVIPS F416 scintillator-based cameras. We compare the results from our new method with published curves.

DOI10.1016/j.jsb.2013.10.016
Alternate JournalJ. Struct. Biol.
PreviewAttachmentSize
Ruskin_JSB2013.pdf2.33 MB