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Akta FPLC

The hardware is located in a deli box controlled at 4-6oC and the software used to control the system is the Unicorn operating system, running on a PC located on the other side of the hallway. Please see the attached user manuals for detailed descriptions of the components and software.

Signups
When reserving the FPLC, please add your name to the calendar above the FPLC computer. Normally, time is reserved in 3-4 day blocks. Please be considerate to other users. As always, repeated failures to use the instrument after reserving time can result in penalties, up to and including banning.

Logging On
The unicorn software has a number of established user accounts. if you are a member of the Grigorieff lab and do not have a user account, please see Mike. if you are not a member of the Grigorieff lab, you may log in using the Guest account.

Columns Available
We currently have the following columns available for use:
Sephacryl S-100
Sephacryl S-200
Superdex 75

Data Output
Unicorn data files can only be read with that software. You can print out a hard copy of your trace and experimental setup on the Rose4gc printer in the computer room. Further processing of data files is not currently supported.

Difficulties
The system can be rather fastidious when rebooted. If you are having problems, talk to Mike.

Comments

Degassing

There are two traditional strategies used to remove problem dissolved gases from chromatographic eluants. Often they
are used in combination to lower the dissolved gases.
1. Subject the solvent to vacuum for 5-10 mins. to remove the gases.
2. Subject the solvent to ultrasonics for 10-15 mins. to remove the gases.

Note that most aqueous-based solvents usually have to be degassed every 24 hours. Also remember that solubility of gases increases as temperature decreases, so eluants should be at instrument temperature prior to degassing.