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A primer to single-particle cryo-electron microscopy

TitleA primer to single-particle cryo-electron microscopy
Publication TypeJournal Article
Year of Publication2015
AuthorsCheng, Y, Grigorieff N, Penczek P  A, Walz T
Refereed DesignationRefereed
JournalCell
Volume161
Issue3
Pagination438 - 449
Date Published04/2015
ISSN00928674
Abstract

Cryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the structure of proteins and macromolecular complexes without the need for crystals. Recent advances in detector technology and software algorithms now allow images of unprecedented quality to be recorded and structures to be determined at near-atomic resolution. However, compared with X-ray crystallography, cryo-EM is a young technique with distinct challenges. This primer explains the different steps and considerations involved in structure determination by single-particle cryo-EM to provide an overview for scientists wishing to understand more about this technique and the interpretation of data obtained with it, as well as a starting guide for new practitioners.

DOI10.1016/j.cell.2015.03.050
Short TitleCell
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Cheng_Cell2015.pdf2.63 MB