Skip to Content

Automatic estimation and correction of anisotropic magnification distortion in electron microscopes

TitleAutomatic estimation and correction of anisotropic magnification distortion in electron microscopes
Publication TypeJournal Article
Year of Publication2015
AuthorsGrant, T, Grigorieff N
Refereed DesignationRefereed
JournalJ Struct Biol
Volume192
Pagination204–208
KeywordsElectron Cryo-Microscopy; Resolution; Magnification Anisotropy; Image Correction
Abstract

We demonstrate a significant anisotropic magnification distortion, found on an FEI Titan Krios microscope and affecting magnifications commonly used for data acquisition on a Gatan K2 Summit detector. We describe a program (mag_distortion_estimate) to automatically estimate anisotropic magnification distortion from a set of images of a standard gold shadowed diffraction grating. We also describe a program (mag_distortion_correct) to correct for the estimated distortion in collected images. We demonstrate that the distortion present on the Titan Krios microscope limits the resolution of a set of rotavirus VP6 images to ~7 Å, which increases to ~3 Å following estimation and correction of the distortion. We also use a 70S ribosome sample to demonstrate that in addition to affecting resolution, magnification distortion can also interfere with the classification of heterogeneous data.

DOI10.1016/j.jsb.2015.08.006
PreviewAttachmentSize
Grant_JSB2015.pdf673.53 KB