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Frealign with phase plate data?

8 replies [Last post]
Axel
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Joined: 20 May 2009

Hi everyone,

I was wondering if there were any plans to implement frealign refinement with phase plate data.

Best,

Axel

Jamon
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Joined: 14 Mar 2013
frealign for VPP dataset

Dear Niko,
I am wondering if there is new version of frealign that can handel VPP data now. I have a few VPP datasets and keen to try out frealign for 3D refinement.

Thanks,
Jian
CryoEM, CBIS, NUS

niko
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Joined: 5 May 2009
A version of Frealign that

A version of Frealign that implements phase shifts is currently being tested and will soon be released.

Jamon
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Joined: 14 Mar 2013
Niko, Thanks for the

Niko, Thanks for the information.
I am also wondering if it's possible just run current Frealign on VPP dataset, only using defocus value but without phase shift? Anything bad would happen?

Regards,
Jian

niko
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Joined: 5 May 2009
Just give it a try.

Just give it a try. Presumably, the CTF will not be correct and you will loose resolution.

niko
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Joined: 5 May 2009
This may well be implemented

This may well be implemented in a future version, but probably not for a few months.

Axel
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Joined: 20 May 2009
I may be interested in

I may be interested in testing out a beta version when this becomes available.

lizkellogg
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Joined: 11 Dec 2015
Hi all, I'm in the same

Hi all,

I'm in the same position as Axel, I would like to try out frealign with support for VPP datasets. Any ETA on when this version will be released?
Thanks

Liz

niko
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Joined: 5 May 2009
We plan to release the new

We plan to release the new version in one to two months from today.