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A dose-rate effect in single-particle electron microscopy

TitleA dose-rate effect in single-particle electron microscopy
Publication TypeJournal Article
Year of Publication2008
AuthorsChen, JZ, Sachse C, Xu C, Mielke T, Spahn CM, Grigorieff N
Refereed DesignationRefereed
JournalJ Struct Biol
Date PublishedJan
ISBN Number1047-8477 (Print)
Accession Number17977018
KeywordsCryoelectron Microscopy/*methods, Image Processing, Computer-Assisted/*methods, Tobacco Mosaic Virus/ultrastructure

A low beam intensity, low electron dose imaging method has been developed for single-particle electron cryo-microscopy (cryo-EM). Experiments indicate that the new technique can reduce beam-induced specimen movement and secondary radiolytic effects, such as "bubbling". The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution.

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