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Using sum_movie to generate aligned stacks?

2 replies [Last post]
oliverbclarke
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Joined: 24 Jul 2015

Hi,

I've used unblur for whole frame alignment, and I'd like to try polishing in relion (but I only generated the aligned sums initially, not aligned stacks).

I still have the shifts files, so I'd like to use them with sum_movie to generate aligned stacks, to speed up the process and ensure that the overall alignment is identical (which I am not 100% sure is guaranteed if I run unblur again to do so).

Thoughts/suggestions?

Cheers

Oli

timgrant
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Joined: 16 Jul 2010
Hi Oli, I've hacked together

Hi Oli,

I've hacked together a version that saves the frames for you - you can download it here :-

http://grigoriefflab.janelia.org/node/5558

I've only done brief tests, so definitely check the output.

Cheers,

Tim

oliverbclarke
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Joined: 24 Jul 2015
Thanks Tim! Apologies for the

Thanks Tim! Apologies for the late reply, I missed your post for some reason, but I've downloaded and tested the modified version now and it seems to work fine. Thanks again!

Cheers
Oli