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Structure of the ciliary axoneme at nanometer resolution reconstructed by TYGRESS

TitleStructure of the ciliary axoneme at nanometer resolution reconstructed by TYGRESS
Publication TypeJournal Article
Year of Publication2018
AuthorsSong, K, Shang Z, Fu X, Lou X, Grigorieff N, Nicastro D
JournalbioRxiv
Abstract

The resolution of subtomogram averages calculated from cryo-electron tomograms (cryo-ET) of crowded cellular environments is often limited due to signal loss in, and misalignment of the subtomograms. In contrast, single-particle cryo-electron microcopy (SP-cryo-EM) routinely reaches near-atomic resolution of isolated complexes. We developed a novel hybrid-method called ''TomographY-Guided 3D REconstruction of Subcellular Structures'' (TYGRESS) that combines cryo-ET with SP-cryo-EM to achieve close-to-nanometer resolution of complexes inside crowded environments. Using TYGRESS, we determined the native 3D structures of the intact ciliary axoneme with up to 12 Å resolution. These results reveal many structures and details that were not visible by cryo-ET. TYGRESS is generally applicable to cellular complexes that are amenable to subtomogram averaging, bringing us a step closer to (pseudo-)atomic models of cells.

URLhttps://www.biorxiv.org/content/early/2018/07/06/363317
DOI10.1101/363317
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Song_2018.pdf3.36 MB