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High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations

TitleHigh-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations
Publication TypeJournal Article
Year of Publication2019
AuthorsJung, J, Grant T, Thomas DR, Diehnelt CW, Grigorieff N, Joshua-Tor L
Refereed DesignationRefereed
JournalProc Natl Acad Sci U S A
Volumein press
Abstract

Noroviruses are a leading cause of food-borne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6-4.1 Å) cryo-electron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7 and GI.1 human norovirus outbreak strain virus-like particles (VLPs). The VLP structures reveal polymorphism between and within genogroups with small, medium and large particle sizes observed. These structures in their assembled three-dimensional context serve as valuable templates for facilitating vaccine formulations.