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Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating

TitleStructure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating
Publication TypeJournal Article
Year of Publication2004
AuthorsFotin, A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T
Refereed DesignationRefereed
JournalNature
Volume432
Pagination649-53
Date PublishedDec 2
ISBN Number1476-4687 (Electronic)
Accession Number15502813
KeywordsAnimals, Auxilins/*chemistry/genetics/*metabolism/ultrastructure, Cattle, Clathrin Heavy Chains/chemistry/metabolism/ultrastructure, Clathrin/chemistry/*metabolism/*ultrastructure, Cryoelectron Microscopy, HSC70 Heat-Shock Proteins, HSP70 Heat-Shock Proteins/metabolism, Models, Molecular, Peptide Fragments/chemistry/metabolism/ultrastructure, Protein Binding, Protein Conformation, Structure-Activity Relationship
Abstract

Clathrin-coated pits invaginate from specific membrane compartments and pinch off as coated vesicles. These vesicles then uncoat rapidly once released. The Hsc70 molecular chaperone effects the uncoating reaction, and is guided to appropriate locations on clathrin lattices by the J-domain-containing co-chaperone molecule auxilin. This raises the question of how a local event such as ATP hydrolysis by Hsc70 can catalyse a global disassembly. Here, we have used electron cryomicroscopy to determine 12-A-resolution structures of in-vitro-assembled clathrin coats in association with a carboxy-terminal fragment of auxilin that contains both the clathrin-binding region and the J domain. We have located the auxilin fragment by computing differences between these structures and those lacking auxilin (described in an accompanying paper). Auxilin binds within the clathrin lattice near contacts between an inward-projecting C-terminal helical tripod and the crossing of two 'ankle' segments; it also contacts the terminal domain of yet another clathrin 'leg'. It therefore recruits Hsc70 to the neighbourhood of a set of critical interactions. Auxilin binding produces a local change in heavy-chain contacts, creating a detectable global distortion of the clathrin coat. We propose a mechanism by which local destabilization of the lattice promotes general uncoating.

URLhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15502813
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