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FREALIGN: high-resolution refinement of single particle structures

TitleFREALIGN: high-resolution refinement of single particle structures
Publication TypeJournal Article
Year of Publication2007
AuthorsGrigorieff, N
Refereed DesignationRefereed
JournalJ Struct Biol
Date PublishedJan
KeywordsAlgorithms, Electron, Image Enhancement, Imaging, Microscopy, Particle Size, Software, Software Design, Three-Dimensional

The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page.

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