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Software Download


Several programs are available for download:

If you encounter problems or need help using these programs, please do not contact us directly in the first instance, use our forum instead.


  1. Grant, T, Rohou A, Grigorieff N.  2018.  cisTEM, User-friendly software for single-particle image processing. eLife. 7(e35383)
  2. Mindell, JA, Grigorieff N.  2003.  Accurate determination of local defocus and specimen tilt in electron microscopy. J Struct Biol. 142:334-47.
  3. Grigorieff, N.  2007.  FREALIGN: high-resolution refinement of single particle structures. J Struct Biol. 157:117–125.
  4. Grigorieff, N.  1998.  Three-dimensional structure of bovine NADH:ubiquinone oxidoreductase (complex I) at 22 Å in ice. J Mol Biol. 277:1033-46.
  5. Wolf, M, DeRosier DJ, Grigorieff N.  2006.  Ewald sphere correction for single-particle electron microscopy. Ultramicroscopy. 106:376-82.
  6. Stewart, A, Grigorieff N.  2004.  Noise bias in the refinement of structures derived from single particles. Ultramicroscopy. 102:67-84.
  7. Sindelar, CV, Grigorieff N.  2012.  Optimal noise reduction in 3D reconstructions of single particles using a volume-normalized filter. J Struct Biol. 180:26-38.
  8. Lyumkis, D, Brilot AF, Theobald DL, Grigorieff N.  2013.  Likelihood-based classification of cryo-EM images using FREALIGN. J Struct Biol. 183:377-388.
  9. Brilot, AF, Chen JZ, Cheng A, Pan J, Harrison SC, Potter CS, Carragher B, Henderson R, Grigorieff N.  2012.  Beam-induced motion of vitrified specimen on holey carbon film.. J Struct Biol. 177:630-637.
  10. Campbell, MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS, Carragher B et al..  2012.  Movies of ice-embedded particles enhance resolution in electron cryo-microscopy. Structure. 20:1823–1828.
  11. Grant, T, Grigorieff N.  2015.  Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6. eLife. 4(e06980):1-19.
  12. Grant, T, Grigorieff N.  2015.  Automatic estimation and correction of anisotropic magnification distortion in electron microscopes. J Struct Biol. 192:204–208.
  13. Rohou, A, Grigorieff N.  2014.  Frealix: model-based refinement of helical filament structures from electron micrographs. J Struct Biol. 186:234–244.
  14. Chen, JZ, Grigorieff N.  2007.  SIGNATURE: a single-particle selection system for molecular electron microscopy. J Struct Biol. 157:168-73.
  15. Grigorieff, N.  2000.  Resolution measurement in structures derived from single particles. Acta Crystallogr D Biol Crystallogr. 56:1270-7.
  16. Sousa, D, Grigorieff N.  2007.  Ab initio resolution measurement for single particle structures. J Struct Biol. 157:201-10.
  17. Sachse, C, Chen JZ, Coureux PD, Stroupe ME, Fändrich M, Grigorieff N.  2007.  High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virus. J Mol Biol. 371:812-35.