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Synthetic 70S ribosome datasets

Frealign benchmark

The attached dataset and parameter files were generated by Dmitry Lyumkis at The Scripps Research Institute for the purpose of evaluating the performance of the maximum-likelihood multi-reference refinement algorithm [1] implemented in Frealign. The image data is stored in MRC/CCP4 format using a pixel size of 2.52 Å, assuming a beam energy of 200 keV and a spherical aberration coefficient of the objective lens of 2.0 mm. The files can also be downloaded from the Scripps NRAMM web page.