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TMV in Ice - Micrographs

TMV

Six images (MRC/CCP4 format) are available for download to test the Spider scripts used for the helical processing of Tobacco Mosaic Virus (TMV, Figure). They are images of TMV in ice at a calibrated magnification of 1.163 Å/pixel. These images were recorded on an FEI F30 at 200 kV with a dose of 15 e-/Å2 and an underfocus between 1.5 and 4.0 µm. Please see [1] for details.

Furthermore, a stack of TMV segments is available for download that was used to reconstruct TMV using Frealign v8.09, which implements a helical symmetry operator. The reconstruction and Frealign parameter files are also included for download below.

Please download one file at a time or use the supplied scripts to download files sequentially. Attempts to download more than one file at a time will cause a Service Temporarily Unavailable message to be displayed.

Image Calibrated mag Scan pixel size Beam voltage Cs Defocus parameters (CTFFIND3)
A00 60190 7.0 µm 200 kV 2 mm 18098.07 Å, 15003.80 Å, 7.47 °
A02 60190 7.0 µm 200 kV 2 mm 16982.86 Å, 12757.80 Å, 14.38 °
A06 60190 7.0 µm 200 kV 2 mm 31700.83 Å, 27762.51 Å, 7.64 °
A08 60190 7.0 µm 200 kV 2 mm 32206.58 Å, 29185.52 Å, 3.06 °
A12 60190 7.0 µm 200 kV 2 mm 40817.04 Å, 36379.68 Å, 10.39 °
A14 60190 7.0 µm 200 kV 2 mm 39239.23 Å, 34608.70 Å, 16.16 °

References